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Affinity Chromatography. Biospecific Sorption–The First - download pdf or read online

By O. Hoffmann-Ostenhof

ISBN-10: 0080226329

ISBN-13: 9780080226323

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Read or Download Affinity Chromatography. Biospecific Sorption–The First Extensive Compendium on Affinity Chromatography as Applied to Biochemistry and Immunochemistry PDF

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Extra resources for Affinity Chromatography. Biospecific Sorption–The First Extensive Compendium on Affinity Chromatography as Applied to Biochemistry and Immunochemistry

Example text

41 Immobilised Coenzymes M H _ ( C H 2 ) 6 — NH (a) OH OH ( C H 2 ) 6 — NH O (b) H g - O — P—O" O" F i g . 1. The s t r u c t u r e s of two adenine-immobilised AMP adsorbents (a) N -(6-aminohexyl)-AMP-agarose and (b) 8-(6-aminohexyl) - A M P - a g a r o s e . Both matrix-bound derivatives fulfill many of the requirements for a "general ligand" and bind a wide range of kinases and dehydrogenases. However, at present, very little comparative data is available to act as a guideline to ascertain which of the two derivatives would be more suitable for the isolation of a particular enzyme.

B i o c h e m 52, 99 (1975). (23) L . E . Aukrust, K . R . N o r u m & B . A . Skalhegg, Affinity chromatography of 3a-hydroxysteroid dehydrogenase from Pseudomonas testosteroni. Use of N, N - d i m e t h y l f o r m a m i d e to prevent hydrophobic interactions between the e n z y m e and the ligand, B i o c h i m . B i o p h y s . A c t a . 438, 13 (1976). (24) P . O ' C a r r a , S. B a r r y & T . Griffin, S p a c e r a r m s in affinity chromatography U s e of hydrophilic a r m s to control or e l i m i n a t e n o n b i o s p e c i f i c adsorption e f f e c t s , F E B S L e t t e r s 4 3 , 169 (1974).

P . T r a y e r , H . R . T r a y e r , D . A . P . S m a l l & R . C. B o t t o m l e y , Preparation of a d e n o s i n e n u c l e o t i d e d e r i v a t i v e s s u i t a b l e for affinity c h r o m a t o g r a p h y , B i o c h e m . J . 139, 609 (1974). (9) H . S c h a l l e r , X . N i i u a a l w i n , F . J . B o n h o e f f e r , C . K u r z & I. N i e t z c h m a n n , Affinity C h r o m a t o g r a p h y of D N A - b i n d i n g e n z y m e s on s i n g l e - s t r a n d e d D N A - a g a r o s e c o l u m n s , E u r .

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Affinity Chromatography. Biospecific Sorption–The First Extensive Compendium on Affinity Chromatography as Applied to Biochemistry and Immunochemistry by O. Hoffmann-Ostenhof


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